SIMULTANEOUS HPLC ANALYSIS OF PALM CAROTENOIDS AND TOCOPHEROLS USING A C-30 COLUMN AND PHOTODIODE ARRAY DETECTOR
作者:
D. Darnoko,
M. Cheryan,
E. Moros,
J. Jerrel,
E.G. Perkins,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 12
页码: 1873-1885
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100100459
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
An HPLC method for simultaneous identification and quantitation of tocopherols and carotenoids in red palm oil was developed. A C-30 column was used in a HPLC equipped with a photodiode detector and with methyl tertiary-butyl ether/methanol/water as the mobile phase. Tocopherols and carotenoids were monitored at 295 nm and 450 nm, respectively. Gradient elution resulted in complete separation of five tocopherols such as α-, δ-, γ-tocopherols and tocotrienols in palm oil in less than 10 minutes. Several (13) carotenoids were also detected and quantitated in red palm oil within 45 minutes.
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