Attenuation of 4-hydroxynonenal-induced cataractogenesis in rat lens by butylated hydroxytoluene
作者:
SrivastavaSanjay K.,
AwasthiSanjay,
WangLifei,
BhatnagarAruni,
AwasthiYogesh C.,
AnsariAseem H.,
期刊:
Current Eye Research
(Taylor Available online 1996)
卷期:
Volume 15,
issue 7
页码: 749-754
ISSN:0271-3683
年代: 1996
DOI:10.3109/02713689609003458
出版商: Taylor&Francis
关键词: 4-Hydroxynonenal;butylated hydroxytoluene;cataract;lipid peroxidation;glutathione S-transferase;rat
数据来源: Taylor
摘要:
Purpose.We have previously shown that 4-hydroxynonenal (4-HNE) causes opacification of cultured rat lenses and that a novel group of glutathione S-transferases (GSTs) exhibit high specific activity towards 4-HNE. Previous studies have shown that t-butylated hydroxy toluene (BHT) induced GSTs in cultured rat lens. Therefore, the purpose of the present studies was to investigate if the opacification of rat lenses exposed to 4-HNE is ameliorated by pre-culturing the lenses in media containing BHT.Methods.Rat lenses were divided into four groups. Groups I and II were controls and groups III and IV were cultured in the presence of 100μM 4-HNE. Groups II and IV were pre-cultured in the media containing 10μ.M BHT for 24 hrs which was designated as 0 time point. Lenses were withdrawn at 24 and 72 h and evaluated for opacification by digital image analysis. Induction of the specific GST isozyme (γGST8-8) was studied in the lens epithelium by immunohistochemical studies.Results.Digital image analysis revealed amelioration in opacification induced by 4-HNE, when the lenses were pre-cultured with BHT. Immunohistochemical studies show that BHT induced GST8_8several folds in the epithelium.Conclusions.These studies indicate that pretreatment with BHT would increase the lens capacity to detoxify 4-HNE by conjugating it with GSH, thus assigning an important detoxication role to this specific GST isozyme in oxidative cataract.
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