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Simultaneous Determination of L- and D-Lactate by Enzyme Modified Carbon Paste Electrodes

 

作者: D. Narasaiah,   U. Spohn,   L. Gorton,  

 

期刊: Analytical Letters  （Taylor Available online 1996）
卷期: Volume 29, issue 2  

页码: 181-201

 

ISSN:0003-2719

 

年代: 1996

 

DOI:10.1080/00032719608000999

 

出版商: Taylor & Francis Group

 

关键词: Amperometry;L-and D-lactate;enzyme modified carbon paste;reagentless biosensors

 

数据来源: Taylor

 

摘要:

Enzyme modified carbon paste electrodes were developed for the determination of L- and D-lactate based on D-lactate dehydrogenase and L-lactate oxidase, respectively. D-lactate dehydrogenase was coimmobilized with a redox mediator bound to a polymer backbone and the cofactor NAD+. L-lactate oxidase and horseradish peroxidase were coadsorbed onto heat treated graphite powder, which was mixed with silicon oil to prepare the corresponding carbon paste.

 

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