Probe-Less Genomic Typing of ARG52 (Type 1 Diabetes-Associated) and Non-Arg52 (Non-Type 1 Diabetes-Associated) HLA-DQA1 Alleles
作者:
SorrentinoRosa,
CostanziSandro,
CascinoIsabella,
TosiRoberto,
期刊:
Autoimmunity
(Taylor Available online 1992)
卷期:
Volume 13,
issue 4
页码: 269-274
ISSN:0891-6934
年代: 1992
DOI:10.3109/08916939209112335
出版商: Taylor&Francis
关键词: HLA typing;Type 1 diabetes
数据来源: Taylor
摘要:
According to recent evidence, the presence of an Arg residue at position 52 in the HLA-DQ alpha chain may confer susceptibility to Type 1 diabetes and thus be possibly used to define quantitatively the genetic risk of this disease. Arg52 and non-Arg52 DQA1 alleles cannot be typed by the conventional cytotoxicity test and they must be distinguished at the genomic level. We describe a simple procedure which discriminates the DQA1 alleles based on the differential electrophoretic migration of the DNA heteroduplexes they form with a reference DNA fragment. A major advantage of this procedure is the fact that no hybridization probe is required. Practically, this typing procedure consists of an electrophoretic run of the products of a selective PCR in polyacrylamide gel.
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