The High Performance Liquid Chromatography and Detection of Phospholipids and Triglycerides. III. Response of the Differential Refractive Index and Ultraviolet Absorption Detector
作者:
BruceJon Compton,
WilliamC. Purdy,
期刊:
Analytical Letters
(Taylor Available online 1984)
卷期:
Volume 17,
issue 16
页码: 1857-1862
ISSN:0003-2719
年代: 1984
DOI:10.1080/00032718408077188
出版商: Taylor & Francis Group
关键词: liquid chromatography;phospholipids;triglycerides;signal ratioing
数据来源: Taylor
摘要:
A liquid chromatographic differential refractive index detector (RI) and an ultraviolet absorption (at 195 nm) detector (UV) were studied for detection of triglyceride (TG) and phospholipid (PL) molecular species. It was found that the log UV/RI detector response ratio was linearly related to the log of the number of conjugated double bonds (degree of unsaturation) of the lipid in a predictable fashion, indicating the usefulness of the combination of both detectors in TG and PL analysis. The detection limit of the RI detector for TG and PL was found to be 5 × 10−7M or as sensitive as the UV detector for saturated TG and PL.
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