The application of FeSO4alone or in combination with gypsum increased the chlorophyll and Fe2+content of leaves and total dry matter production of sugarcane grown on saline calcareous loam soil irrigated with saline or non‐saline water. Treatment of sugarcane with FeSO4independent of gypsum significantly increased leaf chlorophyll which was strongly and positively correlated with leaf Fe2+, DTPA extractable Fe of soil and. dry matter production.