SUMMARYThe removal and grooming behaviour ofApis mellifera intermissaagainstVarroa jacobsoniwas investigated in Tunisia. Workers in 15 test colonies detected and removed up to 75% of artificially infested brood and removed up to 97%-99% of freeze-killed brood in each of two trials. Likewise,A. m. intermissaactively groomed offV. jacobsoni, as shown by a great number of injured mites dropping from naturally infested colonies. Both grooming and removal activities ofA. m. intermissaprovide evidence for active mechanisms of resistance againstV. jacobsoni.