Banding Human Chromosomes Using a Combined C-Banding-Fluorochrome Staining Technique
作者:
BellaJ. L,
GosálvezJ.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1994)
卷期:
Volume 69,
issue 5
页码: 243-248
ISSN:1052-0295
年代: 1994
DOI:10.3109/10520299409106295
出版商: Taylor&Francis
关键词: cytogenetics;chromosome banding;C-banding;human chromosomes;fluorescence microscopy
数据来源: Taylor
摘要:
Slides pretreated for C-banding and stained with DAPI or CMA3 show different banding patterns in human metaphase chromosomes compared to those obtained with either standard Giemsa C-banding or fluorochrome staining alone. Human chromosomes show C-plus DA-DAPI banding after C-banding plus DAPI and enhanced R-banding after C-banding plus Chromomycin A3 staining. If C-banding preferentially removes certain classes of DNA and proteins from different chromosome domains, C-banding pre-treatment may cause a differential DNA extraction from G- and R-bands in human chromosomes, resulting in a preferential extraction of DNA included in G-bands. This hypothesis is partially supported by the selective cleavage and removal of DNA from R-bands of restriction endonuclease HaeIII with C-banding combined with DAPI or Chromomycin A3 staining. Structural factors relating to regional differences in DNA and/or proteins could also explain these results.
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