Determination of 3, 4‐dihydroxyphenylglycol (DHPG) by HPLC with coulometric detection, and correlation with 3‐methoxy‐4‐hydroxyphenylglycol (MHPG) in human plasma
作者:
F. Karege,
J.‐M. Gaillard,
R. Tissot,
J.‐M. Azorin,
M. Valli,
期刊:
Biomedical Chromatography
(WILEY Available online 1987)
卷期:
Volume 2,
issue 1
页码: 30-33
ISSN:0269-3879
年代: 1987
DOI:10.1002/bmc.1130020109
出版商: John Wiley&Sons, Ltd.
数据来源: WILEY
摘要:
AbstractA method for determining plasma 3,4‐dihydroxyphenylethyleneglycol (DHPG), a central noradrenaline (NA) metabolite, is described. The method used HPLC with dual coulometric detection set in screen mode of operation. The isolation of DHPG and related catecholamines (noradrenaline, dopamine and dihydroxybenzylamine) was performed on acid washed alumina extracted with 0.2MHClO4containing EDTA (0.2%), and reduced glutathione as stabilizer. A reversed phase column with an eluting system containing 0.025Mcitric acid‐sodium hydrogen phosphate buffer in the ratio 3:2 (v:v) and 5% methanol was used. The experimental results of plasma DHPG levels compared favourably with the results from the literature, and a positively significant correlation with plasma MHPG was found. This method could be used as an alternative in central NA assessm
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