SUMMARYGolgi vesicles from the tips of growing pollen tubes ofPetuniawere isolated on a discontinuous sucrose gradient and compared with those in vivo by means of EM techniques. The results indicate that the isolation procedure does not alter the morphology of the Golgi vesicles. The isolation technique developed renders it possible to analyse biochemically the contents of Golgi vesicles in relation to cell wall synthesis in germinating pollen.