IMMUNOPRECIPITATION OF BIOSYNTHETICALLY LABELLED, STAGE‐SPECIFIC PROTEINS FROM CULTUREDPLASMODIUM FALCIPARUMUSING INHIBITORY HUMAN SERA
作者:
Peter Myler,
Suying Chiu,
Jacqueline Upcroft,
Daniel Castelino,
Chev Kidson,
期刊:
Australian Journal of Experimental Biology and Medical Science
(WILEY Available online 1982)
卷期:
Volume 60,
issue 1
页码: 91-96
ISSN:0004-945X
年代: 1982
DOI:10.1038/icb.1982.7
出版商: Nature Publishing Group
数据来源: WILEY
摘要:
SummaryProteins from synchronized continuous cultures ofPlasmodium falciparumwere biosynthetically labelled with [35S]methionine at ring, trophozoite, and schizont stages. Several proteins appeared to be synthesized predominantly at one stage only, especially the schizont stage. Immunoprecipitation using inhibitory human sera revealed that a variety of proteins, from all stages of parasite growth, were recognized. More proteins, especially those with high molecular weight, were precipitated from the schizont stage than from ring and trophozoite stages, but two major proteins (MW ∼ 70,000 daltons and ∼ 45,000 daltons) were recognized in all three stages.
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