Cytopathological Changes Induced by Cadmium-Exposure in Canine Proximal Tubular Cells: A Cytochemical and Ultrastructural Study
作者:
Tetsuo Hamada,
Akihide Tanimoto,
Shigetoshi Iwai,
Hitoshi Fujiwara,
Yasuyuki Sasaguri,
期刊:
Nephron
(Karger Available online 1994)
卷期:
Volume 68,
issue 1
页码: 104-111
ISSN:1660-8151
年代: 1994
DOI:10.1159/000188227
出版商: S. Karger AG
关键词: Apoptosis;Cadmium;Cytochemistry;Cytopathology;Proximal tubules
数据来源: Karger
摘要:
A highly sensitive cytochemical method for demonstrating intracellular Cd using 8-hydroxyquinoline was developed and applied in the cytopathoiogicai study of primary-cultured renal tubular cells from beagle kidneys. The Cd-8-hydroxyquinoline emitted a yellowish-green fluorescence which first appeared in the cytoplasm within 30 min and in the nucleus about 60-90 min after exposure to 100 mM CdCl2. It was noteworthy that intranuclear Cd was stained in the nucleolar regions. The sensitivity of the cytochemical method for Cd was estimated to be about 1.0 pg Cd/cell. Ultrastructural features of the dead cells were consistent with those of apoptosis. We conclude that Cd absorbed by proximal tubular cells rapidly reaches to the nuclei and affects nuclear as well as cytoplasmic metabolism.
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