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Design of a fast voltage clamp for biological membranes, using discontinuous feedback

 

作者: R. Brennecke,   B. Lindemann,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1974)
卷期: Volume 45, issue 5  

页码: 656-661

 

ISSN:0034-6748

 

年代: 1974

 

DOI:10.1063/1.1686708

 

出版商: AIP

 

数据来源: AIP

 

摘要:

The construction of a voltage clamp with discontinuous feedback is described. Its main feature is a pulsed membrane current with an amplitude proportional to the voltage error, which is determined by integration in the pulse intervals. A current pulse of 8 &mgr;sec length repeating every 24 &mgr;sec is used. Gain switching assures a fast response to large errors, as well as high steady state accuracy. Membrane voltage changes by 150 mV in 8 &mgr;sec to 1% of the final value. 50 &mgr;sec after such a step, a change of resistive membrane current can be read reliably. Performance is demonstrated with equivalent networks of biological membranes and with applications to black lipid membranes and epithelial membranes.

 

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