Design of a fast voltage clamp for biological membranes, using discontinuous feedback
作者:
R. Brennecke,
B. Lindemann,
期刊:
Review of Scientific Instruments
(AIP Available online 1974)
卷期:
Volume 45,
issue 5
页码: 656-661
ISSN:0034-6748
年代: 1974
DOI:10.1063/1.1686708
出版商: AIP
数据来源: AIP
摘要:
The construction of a voltage clamp with discontinuous feedback is described. Its main feature is a pulsed membrane current with an amplitude proportional to the voltage error, which is determined by integration in the pulse intervals. A current pulse of 8 &mgr;sec length repeating every 24 &mgr;sec is used. Gain switching assures a fast response to large errors, as well as high steady state accuracy. Membrane voltage changes by 150 mV in 8 &mgr;sec to 1% of the final value. 50 &mgr;sec after such a step, a change of resistive membrane current can be read reliably. Performance is demonstrated with equivalent networks of biological membranes and with applications to black lipid membranes and epithelial membranes.
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