Recombinant single‐chain antibodies againsts‐Triazines
作者:
karl kramer,
Bertold Hock,
期刊:
Food and Agricultural Immunology
(Taylor Available online 1996)
卷期:
Volume 8,
issue 2
页码: 97-109
ISSN:0954-0105
年代: 1996
DOI:10.1080/09540109609354908
出版商: Taylor & Francis Group
关键词: ELISA;immunomagnetic separation;recombinant antibody;single‐chain Fv;s‐triazines
数据来源: Taylor
摘要:
Recombinant antibody (RAb) technology is expected to play an essential role in future food and environmental immunoanalysis. The RAb approach is not yet, however, considered as a standard method, mainly due to technical reasons. In order to establish a feasible protocol for the production of hapten specific RAbs, the essential steps of the procedure are considered in detail, especially the polymerase chain reaction amplification of antibody coding genes and the utilization of immunomagnetic selection of phages displaying selective RAbs. The synthesis of functional recombinant single‐chain Fv against s‐triazine herbicides is used as an example. Expressed RAbs are characterized by immunoassay. Although RAb‐based calibration curves were shifted toward higher concentrations by one order of magnitude, the cross‐reactivity pattern of RAbs corresponded to the original monoclonal antibodies. Sequences of different RAb clones derived from the same hybridoma line only differed in a single silent mutation. This confirms the reliability of the RAb procedure.
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