Electron beam generation from a multi‐gap pseudospark discharge was investigated. A pseudospark‐sourced electron beam has two phases, an initial hollow cathode phase (HCP) beam followed by a conductive phase (CP) beam. The beam brightness was measured by a field‐free collimator to be 109and 1011Am−2rad−2for the hollow cathode phase (HCP) beam and the conductive phase (CP) beam respectively. The initial HCP beam from an eight‐gap pseudospark discharge was applied in a Cherenkov interaction between the electron beam and the TM01mode of a 60‐cm long alumina‐lined waveguide. It was found experimentally that significant microwave radiation was generated only when the dielectric was present in the interaction space. If there was no dielectric in the cylindrical waveguide, then a very small background microwave output was detected even when the guide B‐field was absent. This demonstrated, in conjunction with the observation that the microwave output signal was independent of the guide magnetic field over the range 0.13 to 0.26 T, that the radiation from the experiment was due to the Cherenkov interaction mechanism. In addition, two components of the microwave pulse were observed corresponding to the two energy components of the electron beam during the pseudospark discharge breakdown. These results demonstrated that the microwave radiation was generated by Cherenkov amplification of the broadband emission from the pseudospark discharge itself. A background signal level of around 100 W was measured in the frequency range 20 – 50 GHz with a percentage of (2.7 ± 0.6)&percent; in the frequency range 25.5 – 28.6 GHz, when the dielectric lining was removed from the maser. The frequency of the microwave output after the Cherenkov maser interaction was measured to be mainly around 25.5 GHz and the dominating mode was identified as being TM01. The duration of the microwave pulse was approximately 80 ns, with a peak power of around 2 ± 0.2 kW. The gain of this amplifier was measured as 29 ± 3 dB. © 2003 American Institute of Physics