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Nanofiltration for Purification of Nucleotide Sugars

 

作者: Gregor Dudziak,   Sven Fey,   Lutz Hasbach,   Udo Kragl,  

 

期刊: Journal of Carbohydrate Chemistry  （Taylor Available online 1999）
卷期: Volume 18, issue 1  

页码: 41-49

 

ISSN:0732-8303

 

年代: 1999

 

DOI:10.1080/07328309908543977

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-β-N-acetylneuraminic acid (CMP-Neu5Ac)1and guanosine-5'-diphospho-α-D-mannose (GDP-Man)2. With appropriate membrances, the retention rate for CMP-Neu5Ac was 0.995 and for GDP-Man 0.983. Both nucleotide sugars were purified on a gram scale with a purity ≥95%.

 

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