Radioimmunoassayable AVT and AVP in Adult Mammalian Brain Tissue: Comparison of Normal and Brattleboro Rats
作者:
A.A. Rosenbloom,
D.A. Fisher,
期刊:
Neuroendocrinology
(Karger Available online 1975)
卷期:
Volume 17,
issue 4
页码: 354-361
ISSN:0028-3835
年代: 1975
DOI:10.1159/000122373
出版商: S. Karger AG
关键词: Radioimmunoassay;Arginine vasotocin;Arginine vasopressin;Brattleboro rat;Pineal;Hypothalamus;Neurohypophysis
数据来源: Karger
摘要:
Arginine vasopressin (AVP) and vasotocin (AVT) were measured by radioimmunoassay in extracts of cerebral cortex, cerebellum, brain stem, pineal, hypothalamus, and neurohypophysis from normal Long-Evans rats. AVP was present in expected concentrations in pituitary and hypothalamus and there was no evidence of its accumulation elsewhere. AVT was not detectable in these tissues (within the limits imposed on our assay by the presence of excessive amounts of AVP) but was easily detectable in pineal tissue with a concentration of 22.4 ± 6.6 µU/gland. Extracts of neurohypophysis and pineal glands from homozygous Brattleboro rats (rats with hereditary hypothalamic diabetes insipidus) revealed the total absence of AVP and AVT. We conclude that the Brattleboro rat is incapable of synthesizing biologically active neurohypophyseal peptides which contain arginine in position
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