Analysis of Retroviral Proteins by Two-Step DNA, Zinc Binding, and Modified Southern-Western Blotting
作者:
V.A. Morozov,
S. Lagaye,
P.O. Ilyinskii,
D.N. Cherepovsky,
K.V. Ilyin,
期刊:
Intervirology
(Karger Available online 1992)
卷期:
Volume 34,
issue 3
页码: 117-123
ISSN:0300-5526
年代: 1992
DOI:10.1159/000150271
出版商: S. Karger AG
关键词: Nucleic acid;Zinc binding;Immunoblotting;Proteins;Retroviruses
数据来源: Karger
摘要:
Using Southern-Western and zinc-blotting techniques, the interactions of single-stranded DNA and zinc with structural proteins of type B, C, and D retroviruses were examined. Besides nucleic capsid proteins of retroviruses known to form complexes with nucleic acids, some other core proteins of type C retroviruses were shown to bind nucleic acids. All nucleic capsid proteins of the examined retroviruses appeared to be also zinc binding. In the present report, we propose two protein-blotting techniques that could be performed on a single nitrocellulose membrane. A first technique allows to detect zinc- and DNA-binding proteins immobilized on the membrane; a second (a modification of Southern-Western blotting) makes it possible to detect DNA-binding proteins followed by immunological re-probing.
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