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Determination of Thromboxane B2by Normal-Phase High-Performance Liquid Chromatography After Pre-Column Derivatization with Fluorescent Reagents

 

作者: R.Ben Gueddour,   M. Matt,   A. Nicolas,   M. Donner,   J.F. Stoltz,  

 

期刊: Analytical Letters  （Taylor Available online 1993）
卷期: Volume 26, issue 3  

页码: 429-443

 

ISSN:0003-2719

 

年代: 1993

 

DOI:10.1080/00032719308016809

 

出版商: Taylor & Francis Group

 

关键词: Thromboxane B2;HPLC;Pre-column derivatization;Fluorescent reagents;Human platelet rich plasma

 

数据来源: Taylor

 

摘要:

Thromboxane B2(TxB2) was determined by high performance liquid chromatography (HPLC) after pre-column derivatization with fluorescent probes. We report the results of two derivatization procedures. The carboxylic group of thromboxane B2was esterified either by 4-bromomethyl-7-methoxycoumarin or by panacyl bromide whereas its alcoholic functions were derivatized using 7-[(chlorocarbonyl) methoxy]-4 methylcoumarin. Characteristics of the method and first results with TxB2released by platelets under pharmacological stimulation are reported.

 

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