Assembly and Antigenicity of Hepatitis B Virus Core Particles
作者:
Maria Seifer,
David N. Standring,
期刊:
Intervirology
(Karger Available online 1995)
卷期:
Volume 38,
issue 1-2
页码: 47-62
ISSN:0300-5526
年代: 1995
DOI:10.1159/000150414
出版商: S. Karger AG
关键词: Nucleocapsid;Macromolecular architecture;Disulfide-linked dimer;Nucleic acid binding;Cooperative assembly;Topology;Xenopusoocytes
数据来源: Karger
摘要:
Recent studies in Xenopus oocytes and other systems have led to an understanding of the HBV capsid, or core particle, assembly process. Nascent HBV core polypeptides rapidly dimerize. Accumulation of free dimers to a signature concentration (∼0.8 µM) then triggers a highly cooperative capsid assembly reaction. This dimer-to-capsid transition is accompanied by a switch from HBe to HBc antigenicity and appears to be nucleated by interaction between core protein and RNA: deletion of a protamine-like RNA binding domain at the C-terminus of the core protein markedly increases the concentration of dimers needed to drive capsid assembly. The simple assembly pathway seen for HBV capsids mirrors that of R17 bacteriopha
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