An Unexpected Behavior of Horseradish Peroxidase in AOT Reversed Microemulsion:UV spectroscopic and Stopped-Flow Studies
作者:
Chengsong Ma,
Ganzuo Li,
Runnan Shen,
Shuben Li,
Hangqing Wang,
期刊:
Journal of Dispersion Science and Technology
(Taylor Available online 1999)
卷期:
Volume 20,
issue 1-2
页码: 425-436
ISSN:0193-2691
年代: 1999
DOI:10.1080/01932699908943800
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Intermediate compound II and III may be formed as a result of the reaction of horseradish peroxidase with H2O2. From the UV spectra, we can see that the stability of the intermediate compounds is worse in reverse micelle than that in aqueous solution. With the increase of the water content, the stability of intermediate compounds gets better, this means that the characteristic of the water in the water pool is near that of bulk water. The results of the stopped-flow experiments show that the formation rate constant and Vinitialof intermediate compound in reverse microemulsion are bigger than that in aqueous solution. However, within die longer reaction time, the disappearance of the intermediate compound appears early in reverse microemulsion than that in aqueous solution. These results are in agreement with the result of the UV experiments.
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