Dexamethasone as a Blocking Agent in Radioimmunoassay of Unextracted Serum 17β-Estradiol and Testosterone
作者:
I. M. Elbanna,
M. H. Gamal,
H. A. El-Asray,
期刊:
Isotopenpraxis Isotopes in Environmental and Health Studies
(Taylor Available online 1991)
卷期:
Volume 27,
issue 1
页码: 38-41
ISSN:0021-1915
年代: 1991
DOI:10.1080/10256019108622461
出版商: Taylor & Francis Group
关键词: dexamethanose;glucocorticoids;estradiol;steroid hormones;radioimmunoassay;accuracy
数据来源: Taylor
摘要:
The use of dexamethasone (DXM) as a generally applicable blocking agent in steroid RIA was investigated. Precision of estradiol (E2) and testosterone RIA systems was much improved after treatment with DXM. Addition of DXM reformed the standard curves made in hormone free serum (HFS) matrix. Accuracy as percent recoveries of E2spiked in HFS were 115.7 ± 71.2 and 107.4 ± 14 for direct and after addition of DXM, respectively. For T assay these values were 54 ± 23.1 and 103.3 ± 9.5. Direct E2and T RIA using DXM in control serum samples of low, medium, and high levels gave comparable values to solvent extraction. DXM was found to be a potent blocking agent in steroid RIA.
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