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α-Amylase Purification and Separation from Gluco-Amylase by Affinity Chromatography on Cross-Linked Amylose(CL-Amylose)

 

作者: HorstD. Schill,   MirceaA. Mateescu,   Traian Bentia,   Aurora Jifcu,  

 

期刊: Analytical Letters  （Taylor Available online 1981）
卷期: Volume 14, issue 17-18  

页码: 1501-1514

 

ISSN:0003-2719

 

年代: 1981

 

DOI:10.1080/00032718108081476

 

出版商: Taylor & Francis Group

 

关键词: cross-linked amylose;α-amylase;glucoamylase;affinity chromatography;separation

 

数据来源: Taylor

 

摘要:

Using a CL-amylose column it is possible to purify α-amylase from other protein contaminants (e.g. albumin, haemoglobin, etc.) and from interfering exoamylases, by its specific retention on CL-amylose. α-Amylase is eluted off the column in the second step by a mixture containing 0.1 M NaCl in acetic acid 0.01 M and CaCl20.015 M. The recoveries of glucoamylase and α-amylase were 74% and 65% respectively. The retention capacity of the stationary phase is 0.5 mg of α-amylase/ml bed volume.

 

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