Video imaging systems are noi radiometrically calibrated, thus it is difficult to obtain quantitative remotely-sensed imagery for natural resource applications. Video camera automatic gain controls (AGC) present potential problems in calibrating video systems for quantitative analysis because they compensate for changing solar illumination conditions. In this experiment aerial video calibrations to ground reflectance standards were compared for AGC turned on and off, The calibrated aerial video was evaluated for guinea grass (Panicum maximum L.) biomass treatments on two dates. Results showed that there was more atmospheric light scattering in the red than in the NIR video band. Light scattering affects could be detected only when the AGC was ofT because when AGC was on light scattering affects were masked. These results also showed that video imagery produced significant correlations with guinea grass biomass that were comparable to ground reflectance measurements.