Plasma membrane reregionalization in cultured mouse hepatocytes
作者:
Jun Watanabe,
Shinsuke Kanamura,
Kazuo Kanai,
期刊:
The Anatomical Record
(WILEY Available online 1986)
卷期:
Volume 214,
issue 1
页码: 1-7
ISSN:0003-276X
年代: 1986
DOI:10.1002/ar.1092140102
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe cytochemical localization of alkaline phophatase (ALPase) activity and the autoradiographic distribution of glucagon receptors were examined in the plasma membrane of cultured mouse hepatocytes. After 24 hours of culture, ALPase activity was exclusively localized on the plasma membrane in areas of cell‐cell contact, and glucagon receptors were more numerous in the plasma membrane at the periphery of re‐formed cell trabeculae. These results indicate that plasma membrane regionalization of hepatocytes, lost by cell isolation, reappeared during culture. The cells maintained this plasma membrane regionalization until 48 hours of culture. By 72 hours of culture, however, ALPase activity was seen on the external surface of all regions of plasma membrane, and the glucagon receptors decreased markedly in number and became scattered in all regions of plasma membrane. Thus, the re‐formed plasma membrane regionalization disappeared in the cells by 72 hours of cu
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