Evaluation of the l5178y mouse lymphoma cell mutagenesis assay: Quality‐control guidelines and response categories
作者:
William J. Caspary,
Y. Jack Lee,
Scott Poulton,
Brian C. Myhr,
Ann D. Mitchell,
Colette J. Rudd,
期刊:
Environmental Mutagenesis
(WILEY Available online 1988)
卷期:
Volume 12,
issue S13
页码: 19-36
ISSN:0192-2521
年代: 1988
DOI:10.1002/em.2860120503
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: mutagenesis;thymidine kinase locus;quality control;data evaluation;mouse lymphoma assay
数据来源: WILEY
摘要:
AbstractA data‐based approach to formulating quality‐control criteria for the mouse lymphoma cell forward mutation assay is described. Quality‐control guidelines for solvent controls, positive controls, and compound‐treated cultures were developed based on analysis of over 800 experiments. Frequency distributions of experimental parameters of control cultures, such as mutant frequencies, cloning efficiencies, and suspension growths, were examined. Cloning efficiency and relative total growth affected the variability only when the test chemical was highly toxic. This information was used to generate the quality‐control criteria, which were applied to an experiment before it was evaluated for a response. The response categories for classifying the effect of test chemicals on the assay system are defined in terms of (1) the statistically significant differences in average mutant frequency between solvent control cultures and cultures exposed to a chemical and (2) the trend of the dose‐relate
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