Evaluation of the EMIT Amitriptyline and Nortriptyline Assays for the Determination of Serum Clomipramine and Desmethylclomipramine
作者:
Albert Fraser,
Wendy Bryan,
Arthur Isner,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1989)
卷期:
Volume 11,
issue 3
页码: 349-353
ISSN:0163-4356
年代: 1989
出版商: OVID
关键词: Antidepressants;Clomipramine;EMIT;High-performance liquid chromatography.
数据来源: OVID
摘要:
Summary: Homogeneous enzyme immunoassay reagents (EMIT) developed for the measurement of amitriptyline and nortriptyline in serum were modified to allow quantitation of clomipramine and desmethylclomipramine. The method was compared to a high-performance liquid chromatographic method. Between-run precision [coefficient of variation (CV)] for clomipramine in the EMIT assay for amitriptyline ranged from 2.6 to 3.2%. For desmethylclomipramine in the nortriptyline assay, the between-run CV ranged from 1.4 to 1.9%. Serum specimens from 43 patients (desmethylclomipramine) and 59 patients (clomipramine) were analyzed by both methods, with good correlation between methods. For clomipramine, recovery ranged from 100 to 102% (0–600 ng/ml range) and was 95–103% for desmethylclomipramine (0–600 ng/ml). The modified EMIT assays offered sufficient reproducibility, accuracy, and correlation with an established method for routine analysis of clomipramine and desmethylclomipramine.
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