Clonal propagation by tissue culture of Afghan pine (PinuseldaricaMedw.) could be useful for improving Christmas tree production and afforestation of semiarid lands in North America. The objectives were (i) to develop organogenesis procedures for clonal propagation and (ii) to evaluate plantlet performance in the field. Shoot production from cotyledon explants was accomplished on solid Shenk and Hildebrandt medium amended with kinetin (11 μM) and benzyladenine (11 μM). Over 100 shoots per clone were obtained with this method. Spontaneous rooting was prevalent in some of the induced shoots. Shoots without root primordia were induced to form roots with naphthalene acetic acid (5 μM), indole butyric acid (10 μM), and benzyladenine (0.22 μM) in 1/2 strength Shenk and Hildebrandt medium. The rate of plantlet elongation in the field was similar to that of seedlings; however, survival of plantlets was lower than that of seedlings. The lower survival of plantlets was directly related to their smaller size at the time of planting. Organogenesis appears to be a very suitable method for mass micropropagation of this environmentally and economically useful tree.