An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV‐1 for the serology of HIV‐1 infections
作者:
Vera Teeuwsen,
John Schalken,
Guido van der Groen,
Ruud van de Akker,
Jaap Goudsmit,
Albert Osterhaus,
期刊:
AIDS
(OVID Available online 1991)
卷期:
Volume 5,
issue 7
页码: 893-896
ISSN:0269-9370
年代: 1991
出版商: OVID
关键词: HIV-1 serology;enzyme immunoassay;gp41;human monoclonal antibody-K14.
数据来源: OVID
摘要:
An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1 -seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIVANT70infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed.
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