Electrode Hybride Pour la Determination de Phosphates et de Polyphosphates. Application aux Problemes Lies a l'Environnement
作者:
C.R. Linders,
B.J. Vincké,
G.J. Patriarche,
期刊:
Analytical Letters
(Taylor Available online 1985)
卷期:
Volume 18,
issue 17
页码: 2195-2208
ISSN:0003-2719
年代: 1985
DOI:10.1080/00032718508067981
出版商: Taylor & Francis Group
关键词: tissue and enzymatic biosensor;acid phosphatase of Solanum tuberosum and glucose oxydase;amperometry of H2O2
数据来源: Taylor
摘要:
The determination of phosphates and polyphosphates has been effected using an hybrid electrode, which consists of a glucose oxydase enzymic membrane and a Solanum tuberosum tissue slice. This membrane, rich in acid phosphatase, catalyses the glucose-6-phosphate hydrolysis. This reaction is quantitatively inhibited by phosphate. Several factors involved in the electrode response, like substrate concentration, pH, ionic strength and type of buffer are discussed in detail. At a 4.10−4M glucose-6-phosphate concentration, the linear ranges of phosphates and polyphosphates are, respectively, 6.10−5M to 1.6.10−3M and 3.10−5M to 1.10−3M. The urinary phosphate contents determinated by this biosensor are in good agrement with those obtained by usual spectrophotometric techniques.
点击下载:
PDF (368KB)
返 回