Studies on DNA polymerases of Xenopus laevis oocytes: subcellular distribution and physical association of DNA polymerase alpha inside the nucleus
作者:
A. Solari,
L. Zourgui,
P. Carvallo,
L. Tarrago‐Litvak,
期刊:
Biology of the Cell
(WILEY Available online 1986)
卷期:
Volume 56,
issue 1
页码: 23-29
ISSN:0248-4900
年代: 1986
DOI:10.1111/j.1768-322X.1986.tb00439.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
The localization of DNA polymerases in Xenopus laevis oocytes and eggs was studied. Oocytes have a high level of DNA polymerase alpha activity detected exclusively in the nuclei, while mitochondria contain all the DNA polymerase activity of the gamma type. No DNA polymerase beta activity is present in the nuclear fraction. Moreover, the beta activity is not present in unfertilized eggs. In oocytes, DNA polymerase alpha is weakly bound to the nucleoplasm. The leakage of the enzyme from whole nuclei can be prevented using polyvinylpyrrolidone, a nuclear pore sealing agent.
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