A new spatial localization technique forinvivohigh‐resolution imaging is presented here. In contrast to other localization techniques that use a series of rf pulses to define a volume of interest, only one rf pulse is utilized in the proposed method for selection of a region to be imaged. Instead of rf pulses for region selection, subencoding gradient pulses are used for the localization together with a convolution process on each phase‐encoding gradient by a set of additional gradients (e.g.,ydirection). Then the 2‐D localization is completed by restricting the bandwidth in the readout direction (e.g.,xdirection). The latter is simply achieved by using a low‐pass filter in the receiver system. By applying this technique on a human body, localizedinvivohigh‐resolution images are obtained for the knee with much improved resolution. 100×100‐μm in‐plane (x,yplane) resolution images obtained from the human knee demonstrate that localizedinvivohigh‐resolution imaging for both human and animals is possible with an in‐plane resolution of below 100 μm.