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A Modified Enzymatic Method for the Estimation of Dihydroxyacetone

 

作者: A.K. Ghosh,   N.S. Vasan,   Sabita Ghosh,  

 

期刊: Analytical Letters  （Taylor Available online 1975）
卷期: Volume 8, issue 12  

页码: 939-947

 

ISSN:0003-2719

 

年代: 1975

 

DOI:10.1080/00032717508059071

 

出版商: Taylor & Francis Group

 

关键词: Dihydroxyacetone;Estimation;Enzymatic method

 

数据来源: Taylor

 

摘要:

A two stage assay procedure has been developed for the estimation of dihydroxyacetone (DHN). DHA is at first phosphorylated to dihydroxyacetone phosphate (DHAP) by glycerokinase (GK) and ATP at pH 8.5. The DHAP formed is then measured enzymatically using glycerol-3P dehydrogenase (GDH) and NADH at pH 7.6. The sensitivity of this method is found to be adequate for both routine assays and kinetic studies.

 

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