Adaptations of Goldner's Masson Trichrome Stain for the Study of Undecalcified Plastic Embedded Bone
作者:
GruberHelen E.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 1
页码: 30-34
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110002
出版商: Taylor&Francis
关键词: bone histology;Goldner's stain;Masson-Goldner's trichrome stain;osteoid
数据来源: Taylor
摘要:
Specialized adaptations for application of Goldner's Masson trichrome stain to plastic embedded undecalcified bone specimens are presented. This stain can be used successfully on methyl-glycol methacrylate, glycol methacrylate and Spurr embedded bones. The stain affords the advantage of good cellular staining due to the hematoxylin component with concomitant sharp discrimination of mature bone matrix which stains green, immature new bone matrix which stains red, and calcified cartilage which stains very pale green. Use of red filters during photomicrography aids in bone-osteoid discrimination in black and white photographs.
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