Determination of Catecholamines in Human Plasma by HPLC with Electrochemical Detection
作者:
S. Javidan,
M.J. Cwik,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 8
页码: 1339-1348
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608006322
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
An assay procedure is described to quantitate low picogram concentrations of catecholamines in human plasma using reversed phase ion-pair HPLC with electrochemical detection. Optimization of chromatographic conditions allows use of samples as small as 0.25 mL. The overall recovery of 78% offers high precision and accuracy at low concentrations. The lower limit of detection is 0.87 pg/mL for epinephrine, 3.5 pg/mL for norepinephrine and 8.3 pg/mL for dopamine.
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