Evaluation of the Pro-Oxidant and Antioxidant Actions of L-DOPA and Dopamine in Vitro: Implications for Parkinson's Disease
作者:
SpencerJeremy P.E.,
JennerAndrew,
ButlerJohn,
AruomaOkezie I.,
DexterDavid T.,
JennerPeter,
HalliwellBarry,
期刊:
Free Radical Research
(Taylor Available online 1996)
卷期:
Volume 24,
issue 2
页码: 95-105
ISSN:1071-5762
年代: 1996
DOI:10.3109/10715769609088005
出版商: Taylor&Francis
关键词: Dopamine;L-DOPA;Free Radicals;Antioxidants;Peroxyl Radicals
数据来源: Taylor
摘要:
The antioxidant and pro-oxidant properties of L-DOPA and dopamine were investigatedin vitro. Both compounds inhibited the peroxidation of ox-brain phospholipids, with IC50values of 8.5μM for dopamine and 450μM for L-DOPA. Dopamine and L-DOPA reacted with trichloromethyl peroxyl radicals (CC13O2) with rate constants of 2.1×107M1s-1and 1.3×107M-1s-1respectively. The effects of dopamine and L-DOPA on iron ion- dependent hydroxyl radical generation from H2O2were c:mplex. In general, low concentrations stimulated OH formation in the presence of ferric-EDTA and, in the case of L-DOPA, ferric-ADP and ferric citrate chelates. Both compounds also reacted with superoxide radical and hypochlor-ous acid. The products of the reaction with HOCI could still inhibit al-antiproteinase and appear to be 'long lived' chloramine-type oxidizing species. Our results suggest that L-DOPA and dopamine might have a complex mixture of pro- and anti- oxidant effects, which could contribute to tissue damage due to oxidative stress in Parkinson's disease and other neurological disorders.
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