Neuronal protection of oligodendrocytes from antibody‐independent complement lysis
作者:
C Agoropoulou,
S Piddlesden,
P Lachmann,
M Wing,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 5
页码: 927-932
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Axon;Complement;Complement inhibitors;Myelination;Oligodendrocytes
数据来源: OVID
摘要:
CULTURED rat oligodendrocytes are lysed by complement via antibody—independent activation of the classical pathway. This susceptibility to complement lysis has been demonstrated to be due to lack of CD59, a complement regulatory protein which inhibits assembly of the membrane attack complex. In this study the effects of homologous and heterologous complement were examined in a co-culture system of rat oligodendrocytes and peripheral neurones, where axonal ensheathment was observed as early as 4 days after the addition of glial progenitors to the neurones. Following exposure to complement, ensheathing oligodendrocytes were markedly less sensitive to antibody-independent but not antibody-dependent complement lysis than were cells grown without neurones. Immunocytochemical data revealed that co-cultured oligodendrocytes remained CD59 negative, but in contrast to oligodendrocytes cultured alone, were negative for C3b when incubated with C7-deficient serum. Taken together these data indicate that the decreased sensitivity of co-cultured oligodendrocytes to complement lysis is not attributed to the increased expression of CD59, but rather in a failure to activate complement. Incubation of oligodendrocytes with neurone-conditioned medium afforded significant protection (68%), against antibody—independent complement attack, suggesting that soluble neuronal factors can protect oligodendrocytes from complement-mediated lysis.
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