Alterations of the RB tumour suppressor gene in hepatocellular carcinoma and hepatoblastoma cell lines in association with abnormal p53 expression
作者:
M. Farshid,
C. C. Hsia,
E. Tabor,
期刊:
Journal of Viral Hepatitis
(WILEY Available online 1994)
卷期:
Volume 1,
issue 1
页码: 45-53
ISSN:1352-0504
年代: 1994
DOI:10.1111/j.1365-2893.1994.tb00061.x
出版商: Blackwell Publishing Ltd
关键词: carcinogenesis;hepatitis B virus;hepatocellular carcinoma;p53;RB;tumour suppressor gene
数据来源: WILEY
摘要:
SUMMARY.Alterations in the expression of the RB tumour suppressor gene were found by Western immunoblot in three of seven hepatocellular carcinoma and hepatoblastoma cell lines. Abnormalities were detected by single‐strand conformation polymorphism (SSCP) within exons 17–21 in RB cDNA from two of these three cell lines and within exons 20–21 in the third cell line. In these three cell lines with abnormal RB expression, abnormal expression of the p53 tumour suppressor gene was also found, apparently the product of a mutant gene. Thus, mutations within the RB gene (or splice‐site mutations with exon‐skipping) and apparent mutations of the p53 gene together may have contributed to the development of three of these tumours or to the establishment of these c
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