Activity of glutathione synthesis enzymes in the rhesus monkey lens related to age: A model for the human lens
作者:
RathbunWilliam B.,
期刊:
Current Eye Research
(Taylor Available online 1986)
卷期:
Volume 5,
issue 2
页码: 161-166
ISSN:0271-3683
年代: 1986
DOI:10.3109/02713688609015104
出版商: Taylor&Francis
数据来源: Taylor
摘要:
The low activity level of lenticular Y-glutamylcysteine synthetase appears to be an evolutionary phenomenon restricted to higher primates. Rapid reduction with age of the activity of both enzymes (γ-glutamylcysteine synthetase and glutathione synthetase) required for glutathione synthesis in the human lens was demonstrated in an earlier study.The activities ofγ-glutamylcysteine synthetase and giutathione synthetase, the two enzymes responsible for glutathione synthesis, were determined in 39 lenses from the rhesus monkey (Macaca mulatta) as a function of age. The ages ranged from 137 day old fetuses to a 34 year old monkey. Glutathione synthetase activity decreased 8-fold (units/g lens), 7-fold (units/mg soluble protein) and 2-fold (units/lens) over the age span studied.γ-Glutamylcysteine synthetase activity decreased 3-fold (units/g lens), 4-fold (units/mg soluble protein) and less than 2-fold (units/lens) over the same age span. A small increase inγ-glutamylcysteine synthetase activity (units/lens) from embryonic lenses to birth and one year of age was followed in later years by a decrease in activity. In adults, the overall ratio of glutathione synthetase activity toγ-glutamylcysteine synthetase activity was 42:1 as compared to 77:1 for the human and 2:1 to 4:1 for common domestic species. The aging study data indicate that the rhesus monkey lenticular glutathione synthesis system appears to be a good model for the human lens enzymic system.
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