Intracellular calcium and extra‐retinal photoreception inAplysiagiant neurons
作者:
A. M. Brown,
M. S. Brodwick,
D. C. Eaton,
期刊:
Journal of Neurobiology
(WILEY Available online 1977)
卷期:
Volume 8,
issue 1
页码: 1-18
ISSN:0022-3034
年代: 1977
DOI:10.1002/neu.480080102
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe early or “instantaneous” current‐voltage relationship for the light‐activated potassium current inAplysiagiant neurons was linear during the first second of illumination. However, the light current was greatly reduced or abolished by prolonged hyperpolarization. It was also greatly reduced by the injection of calcium EGTA buffers having calcium activities of 5.6 × 10−8Mand simulated by injecting buffers with calcium activities of 2.8–5.6 × 10−7M. Removal of calcium from the extracellular fluid had no effect. Both the light‐and calcium‐activated outward potassium currents were reduced by tetra‐ethylammonium (TEA) ions. The light current was not affected by substituting rubidium for potassium nor by substituting either lithium or Tris for sodium. The calcium‐activated potassium current persisted when the neuron was cooled to 5°C. However, the light response could no longer be elicited. Light hyperpolarizesAplysianeurons probably by increasing intracellular calcium activity two‐to six‐fold which activates a membrane potassium conductance. Calcium levels appear to be restored within the cell and are energy dependent. The light‐activated release of calcium is inhibited by cooling. The body wall ofAplysiatransmits enough visible or 500‐nm light to hyperpolarize someAplysiagiant neurons under ambient conditions. These neurons may be involved in the extraretinal light e
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