Combined Bromodeoxyuridine Immunohistochemistry and Masson Trichrome Staining: Facilitated Detection of Cell Proliferation in Viable vs. Infarcted Myocardium
作者:
LazarousDaisy F.,
ShouMatie,
UngerEllis F.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 5
页码: 253-255
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110032
出版商: Taylor&Francis
关键词: bromodeoxyuridine immunohistochemistry;Masson trichrome counterstain;myocardium;myocardial infarction
数据来源: Taylor
摘要:
Cells in the S-phase of the cell cycle can be identified in tissue sections by im-munohistochemical localization of the thymidine analogue bromodeoxyuridine (BrdU). Generally, a single counterstain is used to visualize the underlying tissue; however, interpretation of morphologic detail is often difficult. We have utilized BrdU to localize proliferating cells in myocardium exposed to angiogenic mitogens. To facilitate identification of labelled nuclei in the context of infarcted vs. viable myocardium, BrdU immunohistochemistry was followed by a modified Mas-son trichrome stain. The time of exposure to the counterstains and the wash protocol were re-revised, permitting clear identification of the labelled brown nuclei against a background of red viable myocardium vs. blue infarct. The combined technique also provides color contrast suitable for computer-based image analysis.
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