Primitive erythropoiesis in chick embryogenesis. III. Effect of FUdR on Hb synthesis
作者:
Graham Lem Campbell,
Harold Weintraub,
Howard Holtzer,
B. H. Mayhall,
期刊:
Journal of Cellular Physiology
(WILEY Available online 1974)
卷期:
Volume 83,
issue 1
页码: 11-18
ISSN:0021-9541
年代: 1974
DOI:10.1002/jcp.1040830103
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA single hematocytoblast in the yolk sac of the chick embryo has been shown previously to give rise on the average to a clone of 128 erythrocytes. Furthermore, in any given generation the erythroid cell synthesizes a characteristic amount of hemoglobin (Hb). In these experiments day 4 embryos were treated with FUdR for 12 hours, and then reversed with thymidine. We have monitored both the passage of these erythroblasts through the cell cycle, and the effect of this perturbation on the Hb content of single cells. As a result of this disruption the amount of Hb synthesized in a given generation can be varied, but the final amount of Hb/cell in the mature erythrocyte is the same as in the untreated controls. Apparently the total amount of the Hb/cell does not in itself influence the passage of the cell through the cycle. The coefficients of variation of the Hb values in the mature erythrocytes from both normal an perturbed embryos are similar.
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