首页   按字顺浏览 期刊浏览 卷期浏览 A Fluorometric Assay ofL-Aspartate: 2-oxoglutarate Aminotransferase in Human Serum with...
A Fluorometric Assay ofL-Aspartate: 2-oxoglutarate Aminotransferase in Human Serum with N-(9-Acridinyl) maleimide

 

作者: Kazuaki Akasaka,   Tateo Suzuki,   Hiroshi Meguro,  

 

期刊: Analytical Letters  （Taylor Available online 1985）
卷期: Volume 18, issue 3  

页码: 357-368

 

ISSN:0003-2719

 

年代: 1985

 

DOI:10.1080/00032718508066138

 

出版商: Taylor & Francis Group

 

关键词: L-asparatate:2-oxoglutarate aminotransferase;enzyme assay;N-(9-acridinyl)maleimide;fluorometry;sulfite

 

数据来源: Taylor

 

摘要:

A new assay ofL-aspartate:2-oxoglutarate aminotransferase (EC 2, 6, 1, 1) (glutamic-oxaloacetic transaminase: GOT) is reported. SubstrateL-aspartate was replaced withL-cysteine sulfinic acid (CSA) which yields sulfite and pyruvate. The enzyme activity was determined by measuring the sulfite with the aid of a fluorescent reagent, N-(9-acridinyl)maleimide (NAM). The method presents a extremely sensitive GOT assay in the range of 4 to 140 U/l using less than 10 μl of human serum.

 

点击下载:  PDF (294KB)



返 回