GROWTH-RELATED SIGNALING IN VASCULAR SMOOTH MUSCLE CELLS OS DEREGULATED BY TCDD DURING THE G0/G1, TRANSITION
作者:
ThomasJ. Weber,
Yang-Yi Fan,
RobertS. Chapkin,
KennethS. Ramos,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1997)
卷期:
Volume 51,
issue 4
页码: 369-386
ISSN:0098-4108
年代: 1997
DOI:10.1080/00984109708984031
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Experiments have been conducted to examine the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on growth-related signaling in vascular smooth muscle cells (SMCs). A 40% reduction of peak DNA synthesis was observed in SMCs only when TCDD was added during the G0/G1, transition of the cell cycle. Enhanced phosphorylation of several endogenous proteins during this period was coincident with increased tyrosine kinase activity as early as 15 min following TCDD challenge. No changes in protein phosphorylation status occurred in cells treated with TCDD during the C,/S transition or during 5 phase. Cotreatment of quiescent SMCs with 10 nM TCDD and serum for 3 h reduced serum-inducible binding activity to a 12-O-tetradecanoyl phorbol 13-acetate responsive element (TRE) by approximately 40%. No alterations of constitutive TRE binding were observed in quiescent SMCs treated with TCDD for up to 5 h. These data show that mitogen-related signaling in vascular SMCs is modulated by TCDD selectively during the G0/G1transition, and these effects influence the growth behavior of these cells.
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