Micro-quantitation of lens myo-inositol by anion exchange chromatography
作者:
DickersonJaime E.,
LouMarjorie F.,
期刊:
Current Eye Research
(Taylor Available online 1990)
卷期:
Volume 9,
issue 2
页码: 201-205
ISSN:0271-3683
年代: 1990
DOI:10.3109/02713689008995207
出版商: Taylor&Francis
数据来源: Taylor
摘要:
A method capable of the determination of pmole quantities of myo-inositol in mgr amounts of tissue by anion exchange chromatography is detailed for use in lens and potentially other tissues. Samples were rendered protein-free through ZnS04/Ba(0H)2precipitation, lyophilized and reconstituted in water just prior to analysis. An aliquot of sample was injected onto an anion exchange column and eluted with a 0.045 M sodium hydroxide mobile phase. Each analysis requires 30 minutes to complete. Average recovery of myo-inositol added to lens sample prior to injection was 100.6%. The coefficient of variation for repeated sample injections was 2.9%. Rabbit lens averaged 11.4μmol/gr wet weight with epithelium containing 8.5μmol/gr wet weight while human lens contained 20.1μmol/gr wet weight and human epithelial cells had 17.5 nmol/mgr protein.
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