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The Nuclear Autoimmune Antigen Ku IS Also Present on the Cell Surface

 

作者: DalzielR. G.,   MendelsonS. C.,   QuinnJ. P.,  

 

期刊: Autoimmunity  （Taylor Available online 1992）
卷期: Volume 13, issue 4  

页码: 265-267

 

ISSN:0891-6934

 

年代: 1992

 

DOI:10.3109/08916939209112334

 

出版商: Taylor&Francis

 

关键词: Ku antigen;FACS analysis;polyclonal antibodies

 

数据来源: Taylor

 

摘要:

Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA 144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.

 

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