Characteristics of the association of nerve growth factor with primary cultures of rat sympathetic neurons
作者:
Edward Hawrot,
期刊:
Journal of Neuroscience Research
(WILEY Available online 1982)
卷期:
Volume 8,
issue 2‐3
页码: 213-224
ISSN:0360-4012
年代: 1982
DOI:10.1002/jnr.490080211
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Nerve Growth Factor (NGF);sympathetic neurons;neuronal cultures
数据来源: WILEY
摘要:
AbstractLong‐term primary cultures of rat sympathetic neurons require NGF for survival and development. The kinetics of the interaction of125I‐NGF with sympathetic neuron cultures suggests the presence of diffusional barriers preventing a determination of true dissociation and association rate constants. Although the observed rate constants do not accurately reflect the microscopic interaction of NGF with receptor, the ratio of the observed rate constants does provide a good estimate of the KD. This value (1 × 10−9M) agrees with earlier steady state measurements of the KD. The association of125I‐NGF with neuronal cultures is temperature‐dependent with internalization and retrograde transport occurring at 37°C. The retrograde transport of125I‐NGF in compartmentalized neuronal cultures is concentration dependent and saturates at about 100 ng/ml (4 × 10−9M). The amount of125I‐NGF accumulated by retrograde transport appears to be increased subsequent to a period of NGF‐starvation. The increase in uptake does not appear to be due to an increase in NGF receptor number since the number of binding sites is not greatly increased
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