The Desheathed Periphery ofAplysiaGiant Neuron. Fine Structure and Measurement of [Ca2+]oFluctuations with Calcium‐selective Microelectrodes
作者:
E. Keicher,
A. Bilbaut,
K. Maggio,
M. L. Hernandez‐Nicaise,
G. Nicaise,
期刊:
European Journal of Neuroscience
(WILEY Available online 1991)
卷期:
Volume 3,
issue 1
页码: 10-17
ISSN:0953-816X
年代: 1991
DOI:10.1111/j.1460-9568.1991.tb00806.x
出版商: Blackwell Publishing Ltd
关键词: calcium selective electrodes;ultrastructure;desheathing
数据来源: WILEY
摘要:
AbstractThe visceral ganglion ofAplysiawas mechanically desheathed after protease softening of the connective tissue to permit the positioning of ion‐selective electrodes in the vicinity of the neuronal membrane. The effects of this treatment on satellite glia and neuronal cytology were observed by electron microscopy. The intracellular alterations were not suggestive of serious membrane damage but the cohesion between glial and neuronal membranes was affected—the glial processes appeared to retract from the trophospongium and in some cases the neuronal membrane was completely naked. The external calcium activity [Ca2+]oat the surface of identified giant neuron, R2, was measured using double‐barrelled calcium‐selective microelectrodes. A decrease of ∼1 mM in [Ca2+]ocould be recorded only during trains of action potentials induced by intracellular depolarizing current injection, and when the electrode was pushed firmly against the neuron surface. A recovery from this decrease in [Ca2+]ocould sometimes, but not always, be observed during the phase of induced neuronal
点击下载:
PDF
(2944KB)
返 回