Study of the Reaction between Nucleic Acids and TB-BPMPHD-CTMAB Complex and its Analytical Application
作者:
Xia Wu,
Jinghe Yang,
Fang Huang,
Min Wang,
Limei Sun,
Guiying Xu,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 12
页码: 2417-2425
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542978
出版商: Taylor & Francis Group
关键词: Nucleic acids;Fluorescence quenching;Tb-BPMPHD-CTMAB complex
数据来源: Taylor
摘要:
The fluorescence quenching of the Tb-BPMPHD-CTMAB by nucleic acids is reported. The maximum fluorescence quenching was obtained at pH5.5, with maximum excitation and emission wavelengths at 300 and 543nm, respectively. Under optimal conditions, the differential value of fluorescence intensity between the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range of 4.0x10−8∼ 1.0x10−5g/ml for fsDNA and 2.5x10−8∼ 6.15x10−6g/ml for yRNA, respectively. The corresponding detection limits are 9.0ng/ml for fsDNA and 5.0ng/ml for yRNA. The method was applied to the determination of DNA (or RNA) in synthetic samples. The results obtained were satisfactory.
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