Alterations in expression of sarcoplasmic reticulum gene in Dahl rats during the transition from compensatory myocardial hypertrophy to heart failure
作者:
Hideki Okayama,
Mareomi Hamada,
Hideo Kawakami,
Syuntaro Ikeda,
Hidetoshi Hashida,
Yuji Shigematsu,
Kunio Hiwada,
期刊:
Journal of Hypertension
(OVID Available online 1997)
卷期:
Volume 15,
issue 12
页码: 1767-1774
ISSN:0263-6352
年代: 1997
出版商: OVID
关键词: sarcoplasmic reticulum;left ventricular hypertrophy;Dahl rat
数据来源: OVID
摘要:
ObjectivesTo clarify whether the functional changes during the transition from compensatory myocardial hypertrophy to failure are associated with changes in sarcoplasmic reticulum gene expression.MethodsWe examined the gene expression of sarcoplasmic reticulum proteins [sarcoplasmic reticulum Ca2+-ATPase (SERCA), phospholamban, calsequestrin and ryanodine receptor] in Dahl salt-sensitive (Dahl-S) rats fed a high-salt (8%) diet from the age of 6 weeks. In-vivo contractile functioning was evaluated using echocardiography, and gene expression of sarcoplasmic reticulum proteins in the left ventricle was analyzed by Northern blotting for each stage of left ventricular hypertrophy.ResultsSERCA messenger RNA (mRNA) levels in Dahl-S rats with compensatory hypertrophy did not change significantly, whereas phospholamban mRNA levels were increased by 61% (P< 0.01), and calsequestrin mRNA levels were increased by 130% (P< 0.01) compared with those in Dahl salt-resistant (Dahl-R) rats. SERCA mRNA levels in Dahl-S rats with decompensated dilatation were decreased by 32% (P< 0.05), whereas levels of phospholamban and calsequestrin mRNA remained unchanged. Ryanodine receptor mRNA levels did not change either with compensatory hypertrophy or with decompensated dilatation.ConclusionsAlterations in expression of sarcoplasmic reticulum gene may be related to changes in systolic and diastolic properties in compensatory hypertrophy and heart failure.
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